Flow-based chemiluminescence microarrays with multilayer plastic foils: cheap, simple, universally applicable

Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

11. Apr 2018
11:00-11:30 h  |  ICM - Internationales Congress Center München Room 4b

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Subjects: 

Analytic

|

Applications

Speaker: 

Dr. Michael Seidel 

(Technical University of Munich)

Type:
Lecture
Speech: English

Most analytical microarrays are manufactured with glass slides by coating step-bystep
a shielding layer with functional groups on surfaces. This shielding layer is
important to prevent unspecific binding and to immobilize biomolecules with high
efficiency. However, these wet-chemical methods consume acids and organic
solvents in high quantities, are very laborious and are difficult to automate. The
utilization of polymer foils instead of glass as a substrate for microarray production is
promising to reduce production costs. Similar to silicon wafer industry, polymer
sheets can be coated with a polymeric paste as shielding layer and antibodies or
DNA can be microdispensed before individual microarray chips are diced by a cutting
plotter at the end.

Here a method for fast and cost-efficient preparation of foil-based microarray chips
from polycarbonate sheets is presented. The polycarbonate surface was
functionalized by screen-printing with a viscous paste of a chemically modified lowcost
polyetheramine on the polycarbonate substrate. After heating and washing with
water a highly hydrophilic surface was obtained. Antibodies, antigens, and
oligonucleotides were directly spotted via micro-contact printing. After incubation, the
sheets were broken apart into individual chips and assembled with a plastic foils
forming a microfluidic channel.

To demonstrate the universal applicability of flow-based plastic microarrays,
antibodies, antibiotics and oligonucleotides were immobilized as capturing probes for
chemiluminescence microarray immunoassays and DNA hybridization assays.
Performing calibration curves we could show that a sandwich microarray
immunoassay for Legionella pneumophila has similar detection limits compared with
glass microarray chips. The polymer paste was so stable on the surface that
regenerable indirect competitive microarray immunoassays for the detection of
antibiotics could be performed. A decrease of 10% of the chemiluminescence signal
was measured after 15 regeneration cycle which was comparable with glass slides.
Finally, we could show that even isothermal amplification of nucleic acids by
heterogeneous polymerase amplification assays can be performed on the same
platform.

Foil-based analytical microarrays are the solution for food safety, water safety and
diagnostics where high qualitative but also cheap consumables are important for
routine applications.

Speaker
Dr. Michael Seidel (Chair of Analytical Chemistry and Water Chemistry and Institute of Hydrochemistry), Technical University of Munich Show Profile

  • Participants

    Speaker
    Dr. Michael Seidel (Chair of Analytical Chemistry and Water Chemistry and Institute of Hydrochemistry), Technical University of Munich Show Profile

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ICM
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Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

Raman microscopic imaging for biomedical applications

11. Apr 2018
13:30-14:00 h  |  ICM - Internationales Congress Center München Room 4b
Speaker: Dr. Christoph Krafft (Leibniz Institute of Photonic Technology)

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Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

Analysis of composition and biological activity of emissions from engines and wood combustion

11. Apr 2018
16:30-17:00 h  |  ICM - Internationales Congress Center München Room 4b
Speaker: Prof. Dr. Ralf Zimmermann (Institute of Chemistry, University of Rostock)

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Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

Environmental issues: how analytical chemistry does help

11. Apr 2018
15:00-15:30 h  |  ICM - Internationales Congress Center München Room 4b
Speaker: Dr. Philippe Garrigues (Université de Bordeaux,)

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Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

Playing tricks on blood cells

11. Apr 2018
14:00-14:30 h  |  ICM - Internationales Congress Center München Room 4b
Speaker: Oliver Hayden (Technical University Munich)

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Apr
11
2018

Analytica Conference > GDCh / Raum 4b > ABC-Spotlight on Bio- and Environmental Analysis

3D Printing of Sub-100 Micrometer Cross-section Microfluidic Devices for Preterm Birth Biomarker Analysis

11. Apr 2018
10:30-11:00 h  |  ICM - Internationales Congress Center München Room 4b
Speaker: Prof. Dr. Adam T. Woolley (Brigham Young University)

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