2D-Fluorescence and UV/Vis Spectroscopy for in-line Bioprocess Monitoring of CHO Cell Cultivations

Apr
10
2018

Analytica Conference > GDCh / Room 3 > Young Analysts in Process Analytics

10. Apr 2018
13:00-13:30 h  |  ICM - Internationales Congress Center München Room 3

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Subjects: 

Analytic

|

Biotechnology & Life Sciences

|

Test and measurement and quality control

|

Pharma & Diagnostics

Speaker: 

Jens Claßen 

(Leibniz Universität Hannover)

Type:
Lecture
Speech: English

The use of spectroscopic sensors for bioprocess monitoring is a powerful tool within the process analytical technology (PAT) initiative of the United States Food and Drug Administration (FDA). In-line measurements are particularly important during cost-intensive manufacturing of biopharmaceuticals in order to facilitate early process fault detection, real time product release and minimize the risk of contamination. Spectroscopic sensors enable simultaneous in-line bioprocess monitoring of various important process variables including biological and chemical variables during the cell cultivation process. The monitoring potential can be increased by multimodal combination of spectroscopic techniques.

In this study, 2D-fluorescence and UV/Vis spectroscopy was used for bioprocess monitoring of mammalian cell cultivations (CHO-K1 cell line) in a stainless steel stirred tank bioreactor. The use of a new 2D-fluorescence sensor with three relevant excitation wavelengths enabled the detection of compounds involved in cellular growth and metabolic activity. In addition, absorption and scattering in the UV/Vis spectra provides information about the cell morphology, total and viable cell count.

Spectral data were analyzed by multivariate data analysis including principal component analysis (PCA) and partial least square (PLS) regression. Results of the PCA demonstrate the comparison of different cultivations and present the number of principal component which are necessary to describe the process variance. PLS regression generates quantitative predictions of process variables such as the cell count and viability for mammalian cell cultivations. Benefits and limitations of both techniques are discussed and possible applications as well as the advantage of combination of different sensors are presented in this work.

Speaker
Jens Claßen, Leibniz Universität Hannover Show Profile

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EntranceEast
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ICM
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Apr
10
2018

Analytica Conference > GDCh / Room 3 > Young Analysts in Process Analytics

Already Analyzing or Still Calibrating? - Demonstration of an online NMR analyzer in pilot scale

10. Apr 2018
14:00-14:30 h  |  ICM - Internationales Congress Center München Room 3
Speaker: Simon Kern (Bundesanstalt für Materialforschung und -prüfung (BAM))

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Apr
10
2018

Analytica Conference > GDCh / Room 3 > Young Analysts in Process Analytics

Optical Spectroscopy and Multivariate Data Analysis for Digital Pathology - What do PAT and Point-of-Care Testing have in common?

10. Apr 2018
12:30-13:00 h  |  ICM - Internationales Congress Center München Room 3
Speaker: Mona Stefanakis (Hochschule Reutlingen)

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Apr
10
2018

Analytica Conference > GDCh / Room 3 > Young Analysts in Process Analytics

A new approach to in-line monitoring of biomass growth by Photon Density Wave spectroscopy as an alternative to established optical PAT

10. Apr 2018
13:30-14:00 h  |  ICM - Internationales Congress Center München Room 3
Speaker: Thomas Schiewe (University of Potsdam)

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