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Optimizing DLS Measurements for Protein Drugs and Biotherapeutics

MÄR
31
2020
31. MÄR 2020

Vortrag Halle A3 Forum Biotech

11:30-12:00 Uhr | Halle A3 A3.560/Forum Biotech

  • Dynamic Light Scattering, or DLS, of proteins can be challenging
  • DLS is a powerful tool for measuring protein size
  • The aim of this study is to demonstrate parameters that can be tweaked to improve measurement quality with monomeric and aggregated monoclonal antibodies.

Themen: Pharma & Diagnostik | Labortechnik | Biotechnologie & Life Sciences | Applikationen

Sprecher: Dr. Daniel Seeman (Brookhaven Instruments)

Format: Vortrag

Sprache: Englisch

DLS is a well know and powerful technique for the size determination of nanoparticles and proteins, its value has been demonstrated in innumerable publications. Proteins however, still represent a challenge by themselves.

Most native proteins are very small in hydrodynamic size, rarely surpassing the 5 – 10 nm, and many of them ranges below 1 nm. Size determination of such small particles is by itself difficult as diffusion coefficient, which is the basis for the calculation of hydrodynamic size, is typically very high thus requiring an advanced correlator with appropriately designed delays layout for determination of the autocorrelation function. Aggregates of natively small proteins however, can easily reach quite large sizes and complicate by this the already challenging measurement.

The Light Scattering signal created by a very low number of aggregates can easily surpass the signal by a large number of small native proteins in the sample. Interpretation of such situation however is crucial in order to fully understand the sample under investigation and return a quality index of it in terms of quality of the purification or of power of representation for further analysis as SAXS and SANS.

This principle is demonstrated with a pharmaceutically relevant protein: monoclonal antibody (or mAb) studied in both monomeric and aggregated states.

Dr. Daniel Seeman

Daniel Seeman is a senior scientist at Brookhaven Instruments, and is responsible for development of new measurement devices and products, as well as applied research and applications support (SLS, DLS, GPC, etc). Daniel completed a postdoc at the NIST Center for Neutron Research (NIST-NCNR) where he helped develop a novel sample environment for use with Small-Angle Neutron Scattering (SANS); his Ph.D. is from the University of Massachusetts-Amherst where he worked with various complex fluids, comprised of colloidal and polymeric components (Polyelectrolytes, Nanoparticles, Surfactants), with an emphasis on static and dynamic light scattering (SLS, DLS) and an undergraduate degree from Stony Brook University, also in chemistry.  

Dr. Daniel Seeman

Brookhaven Instruments

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Dr. Daniel Seeman

Daniel Seeman is a senior scientist at Brookhaven Instruments, and is responsible for development of new measurement devices and products, as well as applied research and applications support (SLS, DLS, GPC, etc). Daniel completed a postdoc at the NIST Center for Neutron Research (NIST-NCNR) where he helped develop a novel sample environment for use with Small-Angle Neutron Scattering (SANS); his Ph.D. is from the University of Massachusetts-Amherst where he worked with various complex fluids, comprised of colloidal and polymeric components (Polyelectrolytes, Nanoparticles, Surfactants), with an emphasis on static and dynamic light scattering (SLS, DLS) and an undergraduate degree from Stony Brook University, also in chemistry.  

Dr. Daniel Seeman

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